Plasmid_Backbone

Part:BBa_K245006:Design

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-09-25)


BB-NIC-II-NC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NotI site found at 70
    Illegal NotI site found at 2125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NgoMIV site found at 2229
    Illegal AgeI site found at 2235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1158


Design Notes

First, vector BB-NIC-II-HisN was cut with EcoRI and XbaI and insert with appropriate part of prefix modified according to standard 37 without His tag was ligated in between.


Source

The basis for BB-NIC-II-NC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.

References