Plasmid_Backbone
Part:BBa_K245006:Design
Designed by: Nika Debeljak Group: iGEM09_Slovenia (2009-09-25)
BB-NIC-II-NC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 53
Illegal XbaI site found at 2179
Illegal XbaI site found at 2223
Illegal SpeI site found at 13
Illegal PstI site found at 77 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal SpeI site found at 13
Illegal PstI site found at 77
Illegal NotI site found at 70
Illegal NotI site found at 2125 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 53
Illegal XbaI site found at 2179
Illegal XbaI site found at 2223
Illegal SpeI site found at 13
Illegal PstI site found at 77 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2119
Illegal XbaI site found at 53
Illegal XbaI site found at 2179
Illegal XbaI site found at 2223
Illegal SpeI site found at 13
Illegal PstI site found at 77
Illegal NgoMIV site found at 2229
Illegal AgeI site found at 2235 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1158
Design Notes
First, vector BB-NIC-II-HisN was cut with EcoRI and XbaI and insert with appropriate part of prefix modified according to standard 37 without His tag was ligated in between.
Source
The basis for BB-NIC-II-NC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.